Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects on Autophagy of Moxibustion at Governor Vessel Acupoints in APP/PS1double-Transgenic Alzheimer's Disease Mice through the lncRNA Six3os1/miR-511-3p/AKT3 Molecular Axis

doi: 10.1155/2022/3881962

Figure Lengend Snippet: Primer sequences.

Article Snippet: PI3K (ab86714, Abcam), AKT3 (bs-5146R, Bioss), mTOR (2972s, CST), LC3B (bs-2912R, Bioss), P62 (18420-1-AP, Triple Eagle), western removal buffer of primary antibody and second antibody (P0025, Beyotime), RIPA cell lysate (P0013B, Beyotime), ECL hypersensitive luminescence kit (34095, Thermo), goat anti-mouse IgG secondary antibody (ZB-2305, Zsbio), goat anti-rabbit IgG secondary antibody (ZB-2301, Zsbio), goat anti-rabbit IgG (FITC) (B029, Ebiogo), sheep serum block (B010, Ebiogo), anti-fluorescence quench blocking agent (containing DAPI) (B024, Ebiogo), TRIzol (15596026, Life Technologies), and hematoxylin (BA-4041, BaSO).

Techniques: Sequencing, Amplification

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects on Autophagy of Moxibustion at Governor Vessel Acupoints in APP/PS1double-Transgenic Alzheimer's Disease Mice through the lncRNA Six3os1/miR-511-3p/AKT3 Molecular Axis

doi: 10.1155/2022/3881962

Figure Lengend Snippet: Expression of the lncRNAs Six3os1, miR-511-3p, and AKT3 in the hippocampus (mean ± SD, n = 6). Note: ∗∗ indicates significant difference versus the control group, at p < 0.01; ## indicates significant difference versus the AD model group, at p < 0.01.

Article Snippet: PI3K (ab86714, Abcam), AKT3 (bs-5146R, Bioss), mTOR (2972s, CST), LC3B (bs-2912R, Bioss), P62 (18420-1-AP, Triple Eagle), western removal buffer of primary antibody and second antibody (P0025, Beyotime), RIPA cell lysate (P0013B, Beyotime), ECL hypersensitive luminescence kit (34095, Thermo), goat anti-mouse IgG secondary antibody (ZB-2305, Zsbio), goat anti-rabbit IgG secondary antibody (ZB-2301, Zsbio), goat anti-rabbit IgG (FITC) (B029, Ebiogo), sheep serum block (B010, Ebiogo), anti-fluorescence quench blocking agent (containing DAPI) (B024, Ebiogo), TRIzol (15596026, Life Technologies), and hematoxylin (BA-4041, BaSO).

Techniques: Expressing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects on Autophagy of Moxibustion at Governor Vessel Acupoints in APP/PS1double-Transgenic Alzheimer's Disease Mice through the lncRNA Six3os1/miR-511-3p/AKT3 Molecular Axis

doi: 10.1155/2022/3881962

Figure Lengend Snippet: Hippocampal expression of PI3K, AKT3, and mTOR proteins (mean ± SD, n = 6). Note: ∗∗ indicates significant difference versus the control group, at p < 0.01; ## indicates significant difference versus the AD model group, at p < 0.01.

Article Snippet: PI3K (ab86714, Abcam), AKT3 (bs-5146R, Bioss), mTOR (2972s, CST), LC3B (bs-2912R, Bioss), P62 (18420-1-AP, Triple Eagle), western removal buffer of primary antibody and second antibody (P0025, Beyotime), RIPA cell lysate (P0013B, Beyotime), ECL hypersensitive luminescence kit (34095, Thermo), goat anti-mouse IgG secondary antibody (ZB-2305, Zsbio), goat anti-rabbit IgG secondary antibody (ZB-2301, Zsbio), goat anti-rabbit IgG (FITC) (B029, Ebiogo), sheep serum block (B010, Ebiogo), anti-fluorescence quench blocking agent (containing DAPI) (B024, Ebiogo), TRIzol (15596026, Life Technologies), and hematoxylin (BA-4041, BaSO).

Techniques: Expressing

Journal: Development (Cambridge, England)

Article Title: Cyclical expression of GDNF is required for spermatogonial stem cell homeostasis

doi: 10.1242/dev.151555

Figure Lengend Snippet: AKT1 and AKT3 are phosphorylated in different types of spermatogonia. (A) Whole-mount wild-type or Tg(Gdnf) tubules co-immunostained for phosphorylated AKT1 Ser473 (P-AKT1) and GFRA1. GFRA+ cells in wild-type testes (arrows) and large clusters of GFRA+ cells in Tg(GDNF) testes (asterisks) are P-AKT1 negative. (B) Whole-mount wild-type or Tg(Gdnf) tubules co-immunostained for phosphorylated AKT3 Ser472 (P-AKT3) and GFRA1. Arrows indicate GFRA1+ P-AKT3+ As and Apr spermatogonia. Arrowheads indicate GFRA1+ P-AKT3+ cluster in Tg(Gdnf) tubules. See also Figs S5 and S6. Scale bars: 100 µm.

Article Snippet: Antibodies used were: mouse PLZF (Santa Cruz Biotech); rat GFRA1 (R&D Systems); rat LIN28A (a gift from Dr Eric G. Moss, UMDNJ, NJ, USA); rabbit SOHLH1 (Abcam); rabbit phospho-S6 (Ser240/244; #2215) and rabbit phospho-AKT1 (S473; D7F10) (Cell Signaling Technology); rabbit phospho-Akt3 (S472, #AP3468a) (Abgent); and mouse KIT (#CBL1360, Millipore) all at 1:200.

Techniques:

Journal: Development (Cambridge, England)

Article Title: Cyclical expression of GDNF is required for spermatogonial stem cell homeostasis

doi: 10.1242/dev.151555

Figure Lengend Snippet: Effect of germ cell-specific deletion of Akt1 on germ cell development. (A-C) No significant change in body weight (A), testis weight (B) or sperm count (C) in 2-month-old mice when Akt1 is deleted in germ cells (n=3). (D) Paraffin wax-embedded sections of testis from control and mutant testis stained with PSA and probed with PLZF antibody. (E) Average PLZF+ cells per tubule (200-250 tubules counted) in control and mutant testis (n=3). (F) Akt1 transcript is significantly (***P=0.002) downregulated in mutant testis (n=3) as determined by qRT-PCR. (G) Absence of P-AKT1 staining in the mutant germ cells. Arrows indicate GFRA1+ P-AKT1+ (double-positive) cells. (H) Detection of P-AKT3 in Akt1-deleted germ cells. Arrows indicate GFRA1+ P-AKT3+ (double-positive) cells. Scale bars: 100 µm.

Article Snippet: Antibodies used were: mouse PLZF (Santa Cruz Biotech); rat GFRA1 (R&D Systems); rat LIN28A (a gift from Dr Eric G. Moss, UMDNJ, NJ, USA); rabbit SOHLH1 (Abcam); rabbit phospho-S6 (Ser240/244; #2215) and rabbit phospho-AKT1 (S473; D7F10) (Cell Signaling Technology); rabbit phospho-Akt3 (S472, #AP3468a) (Abgent); and mouse KIT (#CBL1360, Millipore) all at 1:200.

Techniques: Mutagenesis, Staining, Quantitative RT-PCR

Journal: BioMed Research International

Article Title: Shenmai Injection Supresses Glycolysis and Enhances Cisplatin Cytotoxicity in Cisplatin-Resistant A549/DDP Cells via the AKT-mTOR-c-Myc Signaling Pathway

doi: 10.1155/2020/9243681

Figure Lengend Snippet: Antibodies used in the experiment.

Article Snippet: p-Akt (Ser473) , 33281 M , Bioss , Mouse , 1 : 500.

Techniques: